predicting apparent passive permeability of caco

(PDF) Prediction of Drug Absorption Using Multivariate

Prediction of Drug Absorption Using Multivariate Statistics. William Egan. IntroductionThe primary goal of the drug discovery and development process is to find a molecule possessing both good pharmacodynamic and good pharmacokinetic properties. Ideally, a new drug should be efficacious and selective, target-tissue (s)-specific, and orally

A General Model for Prediction of Caco2 Cell Permeability

In order to deal with current issues of data quantity and quality the wellestablished Caco2 cell model was used to generate accurate permeability data of apparent passive transport for a large set of structurally diverse compounds. PLS statistics was used to correlate calculated descriptors to log P app. This new prediction model for Caco CACO-2 cell lines in drug discovery- An updated perspectiveFeb 05, 2010 · CACO-2 Cell Line/Cell Culture and Transport Experiments. By virtue of the merits stated in the Table-1 CACO-2 cell line is most widely used to screen intestinal permeability, to know drug absorption, and to study passive and active diffusion transport of drug molecules.. Methodology [], []Cell lines used for screening are should be precultivated.

Caco-2 Permeability Assay - cyprotex

The Caco-2 permeability screen is considered to be more representative of human absorption in vivo than PAMPA (parallel artificial membrane permeability assay). PAMPA solely provides a measure of passive diffusion whereas the Caco-2 model provides better prediction of the human absorption for compounds which display active uptake or efflux or pass through the membrane via the paracellular Current Projects - Helmholtz-Centre for Environmental Bittermann, Kai, and Kai-Uwe Goss. 2017. Predicting Apparent Passive Permeability of Caco-2 and MDCK Cell-Monolayers:A Mechanistic Model. PLoS ONE 12(12):120. Ebert, Andrea, Christof Hannesschlaeger, Kai-Uwe Goss, and Peter Pohl. 2018. Passive Permeability of Planar Lipid Bilayers to Organic Anions. Biophysj 115(10):193141.

Estimation of ADME Properties in Drug Discovery

100%) absorbed drugs. Thus, Caco-2 permeability is a valuable index for assessing oral absorption of compounds, which, in turn, calls for methods for predicting chemical Caco-2 permeability. 3. Table 1. Summary of Several Previously Published Caco-2 Permeability Prediction Investigations. Year Authors Method Software Descriptors 1996 Palm . et Evaluation of Optimized Caco-2 Permeability Prediction:logP 0 = -2.8, optimal pH region:6.0 7.5. Oxycodone showed excellent agreement between membrane permeability in Double-Sink PAMPA and transcellular (A B) Caco-2 permeability, see Figure 7. Analysis reveals that 60% of

How Well Can the Caco-2/MadinDarby Canine Kidney

The study aimed to predict effective human jejunal permeability (Peff) using a biophysical model based on parametrized paracellular, aqueous boundary layer, and transcellular permeabilities, and the villus-fold surface area expansion factor (kVF). Published human jejunal data (119 Peff, 53 compounds) were analyzed by a regression procedure incorporating a dual-pore size paracellular model. In Silico Prediction of PAMPA Effective Permeability Using In fact, a variety of in vivo, ex vivo, and in vitro assay systems have been devised to predict the small intestine absorption , of which cell-based assays Madin-Darby Canine Kidney (MDCK) cells and human colon adenocarcinoma derived cell line (Caco-2) and cell-free parallel artificial membrane permeability assay (PAMPA) have been widely

Intestinal absorption of S-nitrosothiols:Permeability and

The evaluation of the bidirectional apparent permeability demonstrated a passive diffusion of the three S-nitrosothiols. GSNO and NACNO preferentially cross the intestinal barrier though the transcellular pathway, while SNAP followed both the trans- and paracellular pathways. Passive diffusion of weak organic electrolytes across Caco Oct 01, 1995 · Predicting apparent passive permeability of Caco-2 and MDCK cell-monolayers:A mechanistic model. Bittermann K, Goss KU. PLoS One, 12(12):e0190319, 27 Dec 2017 Cited by:3 articles PMID:29281711 PMCID:PMC5744993. Free to read & use

Permeation of Insulin, Calcitonin and Exenatide across

Feb 27, 2013 · Objectives Caco-2 monolayers are one of the most widely used in vitro models for prediction of intestinal permeability of therapeutic molecules. However, the conventional Caco-2 monolayer model has several drawbacks including labor-intensive culture process, unphysiological growth conditions, lack of reproducibility and limited throughput. Here, we report on the use of 3-day Caco Predicting apparent passive permeability of Caco-2 and Predicting apparent passive permeability of Caco-2 and MDCK cell-monolayers:A mechanistic model . By Kai Bittermann and Kai-Uwe Goss. Cite . BibTex; Full citation; Publisher:Public Library of Science (PLoS) Year:2017. DOI identifier:10.1371/journal.pone.0190319. OAI identifier:Provided by:

Prediction of Membrane Permeability to Peptides from

Relationships between the dynamic molecular surface properties and intestinal epithelial permeability, as determined in Caco-2 cell monolayers, were used to develop a model for prediction of the transmembrane permeability to the oligopeptide derivatives. Results. A theoretical model was derived which takes both the polar and non-polar part of the dynamic molecular surface area of the Recent Advances in Computational Prediction of Drug retical models to predict drug absorption-related properties, such as intestinal absorption, Caco-2 permeability, and blood-brain partitioning are reviewed. The important physicochemical properties used in the prediction of drug absorp-tion, and the relevance of predictive models in the evaluation of passive drug absorption are discussed.

Tutorial Predicting human pharmacokinetics from

(apparent permeability, 10-6 cm/s) obtained from the Caco-2 permeability study is frequently used instead of P eff because both are closely correlated [2], and the in vitro Caco-2 study is much simpler than the endoscopic balloon study to obtain the P eff in humans. The correlation between P app and P eff is shown in Fig. 4. In summary, P app from Which invitro Screens Guide the Prediction of Oral cial membranes have been compared to Caco-2 cells (Ca-menisch et al. 1997), and for passive diffusion found to behave very similar. This was the basis for the development of a parallel articial membrane permeation assay (PAM-PA) for rapid prediction of transcellular absorption poten-tial (Kansy et al. 1998). In this system, permeability

Predicting apparent passive permeability of Caco-2 and

Dec 01, 2017 · Predicting apparent passive permeability of Caco-2 and MDCK cell-monolayers:A mechanistic model Bittermann, Kai; Goss, Kai-Uwe; Abstract. Publication:PLoS ONE. Pub Date:December 2017 DOI:10.1371/journal.pone.0190319 Bibcode:2017PLoSO..1290319B full text sources